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goat anti nanog  (R&D Systems)


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    Structured Review

    R&D Systems goat anti nanog
    Goat Anti Nanog, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+nanog/Human+Nanog+Antibody/pm41927580-280-71-74
    Average 94 stars, based on 412 article reviews
    goat anti nanog - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described20,21.

    Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described , .

    Staining:

    Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described20,21.

    Article Title: Post-replicative initial expression of PAX6 during neuroectoderm differentiation
    Article Snippet: .. The primary or secondary antibodies used for staining included rabbit anti-PAX6 (Cell Signaling Technology, #60433), mouse anti-OCT3/4 (BD Biosciences, #560307), goat anti-NANOG (R&D Systems, AF1997), mouse anti-TUJ1 (R&D Systems, #MAB1195), donkey anti-rabbit IgG (H + L) antibody Alexa FluorTM 488 (Invitrogen, #A32790), donkey anti-rabbit IgG (H + L) antibody with Alexa FluorTM 647 (Invitrogen, #A32795), and donkey anti-rabbit IgG (H + L) antibody with Alexa FluorTM 594 (Invitrogen, #A32754). ..

    Article Title: Post-replicative initial expression of the cell fate regulator PAX6 during neuroectoderm differentiation
    Article Snippet: .. The primary or secondary antibodies used for staining included rabbit anti-PAX6 (Cell Signaling Technology, #60433), mouse anti-OCT3/4 (BD Biosciences, #560307), goat anti-NANOG (R&D Systems, AF1997), mouse anti-TUJ1 (R&D Systems, #MAB1195), donkey anti-rabbit IgG (H+L) antibody Alexa FluorTM 488 (Invitrogen, #A32790), donkey anti-rabbit IgG (H+L) antibody with Alexa FluorTM 647 (Invitrogen, #A32795), and donkey anti-rabbit IgG (H+L) antibody with Alexa FluorTM 594 (Invitrogen, #A32754). ..

    Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described , .

    Article Title: Post-replicative initial expression of PAX6 during neuroectoderm differentiation.
    Article Snippet: .. The primary or secondary antibodies used for staining included rabbit anti-PAX6 (Cell Signaling Technology, #60433), mouse antiOCT3/4 (BD Biosciences, #560307), goat anti-NANOG (R&D Systems, AF1997), mouse anti-TUJ1 (R&D Systems, #MAB1195), donkey anti-rabbit IgG (H+ L) antibody Alexa FluorTM 488 (Invitrogen, #A32790), donkey anti-rabbit IgG (H+ L) antibody with Alexa FluorTM 647 (Invitrogen, #A32795), and donkey antirabbit IgG (H+ L) antibody with Alexa FluorTM 594 (Invitrogen, #A32754). ..

    Marker:

    Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described20,21.

    Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
    Article Snippet: .. To confirm the pluripotency of CRISPR–Cas9-edited human iPS cell lines, the cells were stained with pluripotency marker antibodies: rabbit anti-OCT4 (ab181557, Abcam, 1:250), goat anti-NANOG (AF1997-SP, R&D Systems, 1:50) and mouse anti-SSEA4 (ab16287, Abcam, 1:250). .. Images were taken using a Nikon ECLIPSE TE2000-U microscope. eSNP-karyotyping was performed for all cell lines used to eliminate potential chromosomal abnormalities, as previously described , .

    Immunocytochemistry:

    Article Title: Whole-genome CRISPR screening identifies genetic modifiers of stem cell-derived islet transplantation.
    Article Snippet: To mount the slides, DAPI Fluoromount-G (SouthernBiotech, 0100-20) was used. .. Primary antibodies were diluted in ICC solution: 1:300 mouse anti-OCT4 (Santa Cruz, Sc-5279), 1:300 goat anti-NANOG (R&D systems, AF1997), 1:300 rat anti-C-peptide (Developmental Studies Hybridoma Bank [DSHB], GN-ID4-S), 1:300 mouse anti-SST (Santa Cruz, sc-55565), 1:300 rabbit anti-GCG (Sigma, 259A-18)), 1:1000 rabbit anti-CHGA (Abcam, ab15160), 1:200 rabbit anti-Ki67 (Abcam, ab16667), or 1:100 mouse anti-Synaptophysin (LSBio, LS-C174787). .. Secondary antibodies were diluted at 1:300 in ICC solution: anti-mouse Alexa Fluor 594 (Invitrogen, A21203), anti-mouse Alexa Fluor 488 (Invitrogen, A11001) anti-goat Alexa Fluor 594 (Invitrogen, A11058), anti-rat Alexa Fluor (Invitrogen, A21208), anti-rabbit Alexa Fluor 594 (Invitrogen, A21207), or anti-rabbit Alexa Fluor 488 (Invitrogen, A21206).

    Article Title: Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation.
    Article Snippet: .. Primary antibodies were diluted in ICC solution at 1:300 unless otherwise indicated: rat anti-C-peptide (GN-ID4-S, Developmental Studies Hybridoma Bank (DSHB)), 1:100 mouse anti-NKX6-1 (F55A12-S, DSHB), goat anti-PDX1 (AF2419, R&D Systems), mouse anti-SOX17 (MAB1924, R&D Systems), mouse anti-GCG (H00002641, Abnova), 1:250 mouse anti-SST conjugated to Alexa Fluor 488 (566032, BD Biosciences), AR TI CL E IN P RE SS rabbit anti-FOXA2 (07-633, MilliporeSigma) or (ab108422, Abcam), mouse anti-OCT-3/4 (sc5279, Santa Cruz Biotechnology), goat anti-NANOG (AF1997, R&D Systems), rabbit anti-Lefty (ab22569, Abcam), mouse anti-Nodal (ab55676, Abcam), rabbit anti-c-Jun (ab40766, Abcam), rabbit anti-TAGLN (ab14106, Abcam), rabbit anti-beta catenin (ab32572, Abcam), mouse antiOTX2 (NBP2-37597, Novus Biologicals), sheep anti-ONECUT1 (AF6277, Novus Biologicals), rabbit anti-BRACHYURY (D2Z3J, Cell Signaling Technology), and rabbit anti-PAX6 (D3A9V, Cell Signaling Technology). .. F-actin was stained directly with 1:500 TRITC-conjugated phalloidin (FAK 100, MilliporeSigma).



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    R&D Systems goat anti human nanog
    (A) Schematic of experimental design. Six donor lines were used for CRISPR-based DNA editing of 66 target genes to introduce a premature stop codon (iSTOP) in batches (∼23 genes and one donor line per batch). The isogenic iPSC lines for 42 edited genes (both edited and unedited) were differentiated into excitatory and inhibitory neurons and co-cultured with mouse astrocytes. The iPSC and iPSC-derived neuron/glia co-cultures were subject to bulk RNA-seq and scRNA-seq, respectively. (B) Representative immunofluorescence staining of iPSC mutant lines for pluripotent stem cell markers <t>(NANOG,</t> SSEA4 and OCT-4). DAPI, nuclei. Scale bar: 100μm. (C) Box plot showing expression of XIST (counts per million reads, CPM) for different editing batches. Each point represents an RNA-seq sample of each line for each batch. Dot color indicates different donor line. The percentage of XIST + samples per donor line is indicated in parenthesis. (D) PCA plot of RNA samples of different isogenic iPSC. Color scheme of each dot is the same as in (C). (E) XIST expression in different isogenic iPSC lines in CW20107 cell lines of the same batch. Non-targeting control (NTC; line V4043) is shown in red. (F) Immunofluorescence staining of the iPSC-differentiated neurons of day 35. MAP2 staining is for neural dendrites. tdTomato staining is for excitatory neurons (infected by AAV-tdTomato before co-cultured with inhibitory neurons). Scale bar: 50μm. (G) Violin Plot showing XIST expression in excitatory (Ex) and Inhibitory (Inh) neurons of different isogenic lines for one example batch using CW20217 as a donor iPSC line for iSTOP base editing. Non-targeting control (NTC; line V4043 and its repeat sample V4043R) is shown in red.
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    R&D Systems 5279 goat anti nanog r d systems cat af1997 mouse anti tuj1 r d systems cat
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    5279 Goat Anti Nanog R D Systems Cat Af1997 Mouse Anti Tuj1 R D Systems Cat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems goat polyclonal anti nanog antibody
    (A) Schematic of experimental design. Six donor lines were used for CRISPR-based DNA editing of 66 target genes to introduce a premature stop codon (iSTOP) in batches (∼23 genes and one donor line per batch). The isogenic iPSC lines for 42 edited genes (both edited and unedited) were differentiated into excitatory and inhibitory neurons and co-cultured with mouse astrocytes. The iPSC and iPSC-derived neuron/glia co-cultures were subject to bulk RNA-seq and scRNA-seq, respectively. (B) Representative immunofluorescence staining of iPSC mutant lines for pluripotent stem cell markers <t>(NANOG,</t> SSEA4 and OCT-4). DAPI, nuclei. Scale bar: 100μm. (C) Box plot showing expression of XIST (counts per million reads, CPM) for different editing batches. Each point represents an RNA-seq sample of each line for each batch. Dot color indicates different donor line. The percentage of XIST + samples per donor line is indicated in parenthesis. (D) PCA plot of RNA samples of different isogenic iPSC. Color scheme of each dot is the same as in (C). (E) XIST expression in different isogenic iPSC lines in CW20107 cell lines of the same batch. Non-targeting control (NTC; line V4043) is shown in red. (F) Immunofluorescence staining of the iPSC-differentiated neurons of day 35. MAP2 staining is for neural dendrites. tdTomato staining is for excitatory neurons (infected by AAV-tdTomato before co-cultured with inhibitory neurons). Scale bar: 50μm. (G) Violin Plot showing XIST expression in excitatory (Ex) and Inhibitory (Inh) neurons of different isogenic lines for one example batch using CW20217 as a donor iPSC line for iSTOP base editing. Non-targeting control (NTC; line V4043 and its repeat sample V4043R) is shown in red.
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    Image Search Results


    (A) Schematic of experimental design. Six donor lines were used for CRISPR-based DNA editing of 66 target genes to introduce a premature stop codon (iSTOP) in batches (∼23 genes and one donor line per batch). The isogenic iPSC lines for 42 edited genes (both edited and unedited) were differentiated into excitatory and inhibitory neurons and co-cultured with mouse astrocytes. The iPSC and iPSC-derived neuron/glia co-cultures were subject to bulk RNA-seq and scRNA-seq, respectively. (B) Representative immunofluorescence staining of iPSC mutant lines for pluripotent stem cell markers (NANOG, SSEA4 and OCT-4). DAPI, nuclei. Scale bar: 100μm. (C) Box plot showing expression of XIST (counts per million reads, CPM) for different editing batches. Each point represents an RNA-seq sample of each line for each batch. Dot color indicates different donor line. The percentage of XIST + samples per donor line is indicated in parenthesis. (D) PCA plot of RNA samples of different isogenic iPSC. Color scheme of each dot is the same as in (C). (E) XIST expression in different isogenic iPSC lines in CW20107 cell lines of the same batch. Non-targeting control (NTC; line V4043) is shown in red. (F) Immunofluorescence staining of the iPSC-differentiated neurons of day 35. MAP2 staining is for neural dendrites. tdTomato staining is for excitatory neurons (infected by AAV-tdTomato before co-cultured with inhibitory neurons). Scale bar: 50μm. (G) Violin Plot showing XIST expression in excitatory (Ex) and Inhibitory (Inh) neurons of different isogenic lines for one example batch using CW20217 as a donor iPSC line for iSTOP base editing. Non-targeting control (NTC; line V4043 and its repeat sample V4043R) is shown in red.

    Journal: bioRxiv

    Article Title: Female iPSC X-chromosome inactivation (XCI) erosion and its transcriptomic effects during CRISPR gene editing and neural differentiation

    doi: 10.64898/2026.02.27.708613

    Figure Lengend Snippet: (A) Schematic of experimental design. Six donor lines were used for CRISPR-based DNA editing of 66 target genes to introduce a premature stop codon (iSTOP) in batches (∼23 genes and one donor line per batch). The isogenic iPSC lines for 42 edited genes (both edited and unedited) were differentiated into excitatory and inhibitory neurons and co-cultured with mouse astrocytes. The iPSC and iPSC-derived neuron/glia co-cultures were subject to bulk RNA-seq and scRNA-seq, respectively. (B) Representative immunofluorescence staining of iPSC mutant lines for pluripotent stem cell markers (NANOG, SSEA4 and OCT-4). DAPI, nuclei. Scale bar: 100μm. (C) Box plot showing expression of XIST (counts per million reads, CPM) for different editing batches. Each point represents an RNA-seq sample of each line for each batch. Dot color indicates different donor line. The percentage of XIST + samples per donor line is indicated in parenthesis. (D) PCA plot of RNA samples of different isogenic iPSC. Color scheme of each dot is the same as in (C). (E) XIST expression in different isogenic iPSC lines in CW20107 cell lines of the same batch. Non-targeting control (NTC; line V4043) is shown in red. (F) Immunofluorescence staining of the iPSC-differentiated neurons of day 35. MAP2 staining is for neural dendrites. tdTomato staining is for excitatory neurons (infected by AAV-tdTomato before co-cultured with inhibitory neurons). Scale bar: 50μm. (G) Violin Plot showing XIST expression in excitatory (Ex) and Inhibitory (Inh) neurons of different isogenic lines for one example batch using CW20217 as a donor iPSC line for iSTOP base editing. Non-targeting control (NTC; line V4043 and its repeat sample V4043R) is shown in red.

    Article Snippet: The following antibodies were used: rabbit anti-Oct4 (1:250) (abcam #ab181557), mouse anti-SSEA4 (1:250; abcam #ab16287), goat anti-Human Nanog (1:20; R&D Systems #AF1997), donkey anti-Rabbit IgG Alexa Fluor Plus 647 (Fisher Scientific #A32795), donkey anti-Mouse IgG Alexa Fluor Plus 594 (Fisher Scientific #A32744), and chicken anti-Goat IgG Alexa Fluor 488 (Fisher Scientific #A21467).

    Techniques: CRISPR, Introduce, Cell Culture, Derivative Assay, RNA Sequencing, Immunofluorescence, Staining, Mutagenesis, Expressing, Control, Infection